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px 458 2sgrna vector  (Addgene inc)


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    Addgene inc px 458 2sgrna vector
    Px 458 2sgrna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px+458+2sgrna+vector/Px458-Cas9n-Trp63-Exon4-2sgRNA+(Plasmid+%2388849)/pmc06643346-41-1-11
    Average 93 stars, based on 1 article reviews
    px 458 2sgrna vector - by Bioz Stars, 2026-09
    93/100 stars

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    Plasmid Preparation:

    Article Title: A New MicroRNA Cluster Involved in the Reprogramming to a Pluripotent State
    Article Snippet: .. The pX-458-2sgRNA vector was obtained by hydrolysis of the pX333 vector (Addgene Plasmid #64073) by XbaI and KpnI restriction endonucleases, isolation and purification of a 444 bp fragment, and insertion of the fragment into the pSpCas9(BB)-2A-GFP (PX458) vector (Addgene Plasmid #48138) hydrolyzed by XbaI and KpnI. .. Rat fibroblasts were cultured at 37 °C and 5% CO 2 in a 1:1 mixture of DMEM and F12 (Lonza) media supplemented with 10% fetal bovine serum (Gibco, USA), GlutaMAX (Gibco), and a mixture of 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco).

    Isolation:

    Article Title: A New MicroRNA Cluster Involved in the Reprogramming to a Pluripotent State
    Article Snippet: .. The pX-458-2sgRNA vector was obtained by hydrolysis of the pX333 vector (Addgene Plasmid #64073) by XbaI and KpnI restriction endonucleases, isolation and purification of a 444 bp fragment, and insertion of the fragment into the pSpCas9(BB)-2A-GFP (PX458) vector (Addgene Plasmid #48138) hydrolyzed by XbaI and KpnI. .. Rat fibroblasts were cultured at 37 °C and 5% CO 2 in a 1:1 mixture of DMEM and F12 (Lonza) media supplemented with 10% fetal bovine serum (Gibco, USA), GlutaMAX (Gibco), and a mixture of 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco).

    Purification:

    Article Title: A New MicroRNA Cluster Involved in the Reprogramming to a Pluripotent State
    Article Snippet: .. The pX-458-2sgRNA vector was obtained by hydrolysis of the pX333 vector (Addgene Plasmid #64073) by XbaI and KpnI restriction endonucleases, isolation and purification of a 444 bp fragment, and insertion of the fragment into the pSpCas9(BB)-2A-GFP (PX458) vector (Addgene Plasmid #48138) hydrolyzed by XbaI and KpnI. .. Rat fibroblasts were cultured at 37 °C and 5% CO 2 in a 1:1 mixture of DMEM and F12 (Lonza) media supplemented with 10% fetal bovine serum (Gibco, USA), GlutaMAX (Gibco), and a mixture of 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco).



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    px 458 2sgrna vector  (Addgene inc)
    93
    Addgene inc px 458 2sgrna vector
    Px 458 2sgrna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px+458+2sgrna+vector/Px458-Cas9n-Trp63-Exon4-2sgRNA+(Plasmid+%2388849)/pmc06643346-41-1-11
    Average 93 stars, based on 1 article reviews
    px 458 2sgrna vector - by Bioz Stars, 2026-09
    93/100 stars
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